Ready-to-Run Pipelines
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NGS Data Analysis for Animal Diagnostics
BASE₂BIO and NGS in Animal Diagnostics
Rapid, accurate identification of infectious agents is essential for effective animal health management—whether for companion animals, livestock, poultry, or aquaculture. Next-generation sequencing (NGS) has transformed what is possible in diagnostic microbiology by enabling comprehensive, untargeted detection of pathogens from a single sequencing run. Yet for many diagnostic laboratories, translating sequencing data into clinically meaningful results remains a significant challenge.
BASE₂BIO bridges that gap with production-ready bioinformatics pipelines designed specifically for animal health diagnostics. Our cloud-based analysis platform transforms raw sequencing data into clear, interactive reports that provide actionable insights for veterinarians, diagnostic laboratories, researchers, and commercial animal health organizations.
Our Pipelines
Non-Targeted Pipeline

Identify viruses, bacteria, fungi, and other microorganisms in a single assay without requiring prior assumptions about what may be present. This comprehensive approach is ideal for investigating unknown or complex disease cases.
Amplicon Pipeline

Analyze targeted genetic regions with high sensitivity to detect and characterize specific organisms or microbial communities. Ideal for applications such as microbiome profiling and targeted pathogen detection. This pipeline handles single-amplicon sequencing, tiled-amplicon sequencing, and multiplexed amplicon panels.
Viral De Novo
WGS Pipeline

Assemble complete viral genomes directly from sequencing data without relying on a reference genome. This enables characterization of novel, highly variable, or previously unrecognized viruses.
Bacterial De Novo
WGS Pipeline

Assemble complete bacterial genomes for detailed characterization of pathogens, including strain typing, virulence factors, plasmids, and antimicrobial resistance markers. Ideal for epidemiology, surveillance, and advanced diagnostic investigations.
*All of our pipelines can use either short read data, long read data, or both as input.
Currently Available Curated Modules



Ready to get started?
If you would like to learn more about our diagnostic pipelines or see an interactive demonstration of our reports, contact us to schedule a personalized demonstration or request a complimentary trial analysis using your own sequencing data.
FAQS
What pipelines are currently available?
Our validated pipelines for for poultry and livestock are actively used by diagnostic laboratories, commercial organizations, and research partners to analyze sequencing data quickly, consistently, and at scale. Click here to learn more about our state-of-the-art pipelines or to see our currently available curated modules.
What is the Hybrid Pipeline Philosophy?
Our hybrid analysis philosophy combines the breadth of untargeted metagenomic sequencing with the precision of curated, organism-specific analyses. Every sample is first evaluated using comprehensive untargeted workflows to characterize the complete microbial population. When pathogens or organisms of interest are detected, our curated taxon-specific modules perform deeper analyses—including phylogenetic characterization, variant analysis, antimicrobial resistance profiling (where applicable), and other organism-specific investigations—to provide a higher level of confidence and biological interpretation. Click here to see how this works.
What makes your data analysis and reports unique?
BASE₂BIO integrates machine learning models into its analysis platform to further enhance diagnostic interpretation. By combining advanced computational methods with curated biological data, these models help prioritize findings, improve confidence in results, and provide meaningful decision support as sequencing technologies and diagnostic applications continue to evolve. In addition, our extensive experience in animal health enables us to deliver more targeted, biologically relevant, and informative analyses.
Click here to view a report and see how we use our probability scoring.
What is the turnaround time from upload to reports received?
From uploading your raw data to our secure site and emailing a completed dataset to receiving your reports, the turnaround time for the production workflows (not the custom developed workflows) is 1-2 business days, with many analyses completed even sooner.
What is the advantage of using metagenomics or non-targeted sequencing in diagnostics?
Through the use of metagenomic sequencing and data analysis, you are able to identfy known pathogens while also detecting unexpected, emerging, or novel microbial agents through this non-targeted analysis. See a sample metagenomics report.
Why do the curated databases make a difference?
Host-specific and organism-specific databases, continuously maintained by our team, improve analytical accuracy while reducing background noise and simplifying interpretation.
What can I expect to see when I receive my reports?
Our reports provide multiple levels of detail—from an overall summary page to in-depth sequence data, phylogenetic analyses, variant information, antimicrobial resistance results, and downloadable supporting data for advanced users. Learn more about our reports.
Can our laboratory or institution have reports branded with our own logo?
Yes. For high-volume diagnostic laboratories and institutions, BASE₂BIO offers custom-branded reports that can be personalized with your organization’s logo. This allows you to provide professional diagnostic reports to your customers while maintaining your own branding. Branded reports are available upon request.
What types of sequencing platforms do you accept?
All sequencing platforms (long and short read)
Who owns the data that I provide to BASE₂BIO for the analysis?
The data is belongs to you, we will only use it for research and development purposes. We can remove it at your request.
How long do you retain sequencing data?
BASE₂BIO retains sequencing data indefinitely, allowing you to access your data whenever needed for future review, reanalysis, or reference.
What are the advantages of outsourcing my bioinformatics?
Scale and Pipeline maintenance. Automated, standardized workflows eliminate the need for extensive in-house bioinformatics expertise while providing consistent, reproducible analyses across projects of any size. Additionally, our pipelines are regularly updated with new reference databases, analytical methods, curated modules, and machine learning enhancements, allowing customers to benefit from ongoing improvements without maintaining their own infrastructure.
What does it look like to get started with BASE₂BIO‘s services?
Begin by filling out our Contact Form and provide us information on what services you are interested or any questions you may have. Once this has been completed, a member of our team will reach out to you to answer your questions or to set up an initial meeting. Depending on the services you are seeking, we will then set up an account for you so that you can immediately begin to upload your raw data. At this time you will also receive instructions and a dataset table that will need to be completed with the data upload. Upon receiving all this information you can expect to receive your report within 24-48 hours. You will be contacted directly by one of our team members throughout the process so that you are aware of what is needed and what to expect.
