Publications

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At BASE₂BIO we are first and foremost, scientists. Collected here is a list of publications co-authored by our team members, either in their current role or in other capacities. For full-text copies of specific articles not available publicly please contact us.
1: Afonso CL. Virulence during Newcastle Disease Viruses Cross Species Adaptation. Viruses. 2021 Jan 15;13(1):110. doi: 10.3390/v13010110. PMID:33467506; PMCID: PMC7830468.
2: Afonso CL, Afonso AM. Next-Generation Sequencing for the Detection of Microbial Agents in Avian Clinical Samples. Vet Sci. 2023 Dec 4;10(12):690. doi: 10.3390/vetsci10120690. PMID: 38133241; PMCID: PMC10747646.
3: Butt SL, Taylor TL, Volkening JD, Dimitrov KM, Williams-Coplin D, Lahmers KK, Miller PJ, Rana AM, Suarez DL, Afonso CL, Stanton JB. Rapid virulence prediction and identification of Newcastle disease virus genotypes using third-generation sequencing. Virol J. 2018 Nov 22;15(1):179. doi: 10.1186/s12985-018-1077-5. PMID: 30466441; PMCID: PMC6251111.
4: Butt SL, Kariithi HM, Volkening JD, Taylor TL, Leyson C, Pantin-Jackwood M, Suarez DL, Stanton JB, Afonso CL. Comparable outcomes from long and short read random sequencing of total RNA for detection of pathogens in chicken respiratory samples. Front Vet Sci. 2022 Dec 1;9:1073919. doi: 10.3389/fvets.2022.1073919. PMID: 36532355; PMCID: PMC9751482.
5: de Oliveira LB, Stanton JB, Zhang J, Brown C, Butt SL, Dimitrov K, Afonso CL, Volkening JD, Lara LJC, de Oliveira CSF, Ecco R. Runting and Stunting Syndrome in Broiler Chickens: Histopathology and Association With a Novel Picornavirus. Vet Pathol. 2021 Jan;58(1):123-135. doi: 10.1177/0300985820969971. Epub 2020 Dec 7. PMID: 33280540.
6: Dimitrov KM, Sharma P, Volkening JD, Goraichuk IV, Wajid A, Rehmani SF, Basharat A, Shittu I, Joannis TM, Miller PJ, Afonso CL. A robust and cost- effective approach to sequence and analyze complete genomes of small RNA viruses. Virol J. 2017 Apr 7;14(1):72. doi: 10.1186/s12985-017-0741-5. PMID: 28388925; PMCID: PMC5384157.
7: Ferreira HL, Taylor TL, Absalon AE, Dimitrov KM, Cortés-Espinosa DV, Butt SL, Marín-Cruz JL, Goraichuk IV, Volkening JD, Suarez DL, Afonso CL. Presence of Newcastle disease viruses of sub-genotypes Vc and VIn in backyard chickens and in apparently healthy wild birds from Mexico in 2017. Virus Genes. 2019 Aug;55(4):479-489. doi: 10.1007/s11262-019-01663-1. Epub 2019 Apr 12. PMID: 30976951.
8: Garcia M, Spatz SJ, Cheng Y, Riblet SM, Volkening JD, Schneiders GH. Attenuation and protection efficacy of ORF C gene-deleted recombinant of infectious laryngotracheitis virus. J Gen Virol. 2016 Sep;97(9):2352-2362. doi: 10.1099/jgv.0.000521. Epub 2016 Jun 9. PMID: 27283114.
9: Kariithi HM, Ferreira HL, Welch CN, Ateya LO, Apopo AA, Zoller R, Volkening JD, Williams-Coplin D, Parris DJ, Olivier TL, Goldenberg D, Binepal YS, Hernandez SM, Afonso CL, Suarez DL. Surveillance and Genetic Characterization of Virulent Newcastle Disease Virus Subgenotype V.3 in Indigenous Chickens from Backyard Poultry Farms and Live Bird Markets in Kenya. Viruses. 2021 Jan 13;13(1):103. doi: 10.3390/v13010103. PMID: 33451125; PMCID: PMC7828601. 10: Kariithi HM, Volkening JD, Leyson CM, Afonso CL, Christy N, Decanini EL, Lemiere S, Suarez DL. Genome Sequence Variations of Infectious Bronchitis Virus Serotypes From Commercial Chickens in Mexico. Front Vet Sci. 2022 Jul 12;9:931272. doi: 10.3389/fvets.2022.931272. PMID: 35903135; PMCID: PMC9315362.
11: Kariithi HM, Christy N, Decanini EL, Lemiere S, Volkening JD, Afonso CL, Suarez DL. Detection and Genome Sequence Analysis of Avian Metapneumovirus Subtype A Viruses Circulating in Commercial Chicken Flocks in Mexico. Vet Sci. 2022 Oct 19;9(10):579. doi: 10.3390/vetsci9100579. PMID: 36288192; PMCID: PMC9612082.
12: Kariithi HM, Volkening JD, Goraichuk IV, Ateya LO, Williams-Coplin D, Olivier TL, Binepal YS, Afonso CL, Suarez DL. Unique Variants of Avian Coronaviruses from Indigenous Chickens in Kenya. Viruses. 2023 Jan 17;15(2):264. doi: 10.3390/v15020264. PMID: 36851482; PMCID: PMC9961390.
13: Kariithi HM, Volkening JD, Alves VV, Reis-Cunha JL, Arantes LCRV, Fernando FS, Filho TF, da Silva Martins NR, Lemiere S, de Freitas Neto OC, Decanini EL, Afonso CL, Suarez DL. Complete Genome Sequences of Avian Metapneumovirus Subtype B Vaccine Strains from Brazil. Microbiol Resour Announc. 2023 Jun 20;12(6):e0023523. doi: 10.1128/mra.00235-23. Epub 2023 May 10. PMID: 37162354; PMCID: PMC10281098.
14: Kariithi HM, Volkening JD, Chiwanga GH, Pantin-Jackwood MJ, Msoffe PLM, Suarez DL. Genome Sequences and Characterization of Chicken Astrovirus and Avian Nephritis Virus from Tanzanian Live Bird Markets. Viruses. 2023 May 25;15(6):1247. doi: 10.3390/v15061247. PMID: 37376547; PMCID: PMC10302499.
15: Kariithi HM, Volkening JD, Chiwanga GH, Goraichuk IV, Msoffe PLM, Suarez DL. Molecular Characterization of Complete Genome Sequence of an Avian Coronavirus Identified in a Backyard Chicken from Tanzania. Genes (Basel). 2023 Sep 23;14(10):1852. doi: 10.3390/genes14101852. PMID: 37895200; PMCID: PMC10606662. 16: Kariithi HM, Volkening JD, Chiwanga GH, Goraichuk IV, Olivier TL, Msoffe PLM, Suarez DL. Virulent Newcastle disease virus genotypes V.3, VII.2, and XIII.1.1 and their coinfections with infectious bronchitis viruses and other avian pathogens in backyard chickens in Tanzania. Front Vet Sci. 2023 Oct 19;10:1272402. doi: 10.3389/fvets.2023.1272402. PMID: 37929287; PMCID: PMC10625407.
17: Kariithi HM, Volkening JD, Afonso CL, Helmy M, Chaudhari PP, Decanini EL. Coding-complete genome sequence of a GI-13 infectious bronchitis virus from commercial chicken in India. Microbiol Resour Announc. 2025 Mar 11;14(3):e0114224. doi: 10.1128/mra.01142-24. Epub 2025 Jan 27. PMID: 39868773; PMCID: PMC11895471.
18: Kariithi HM, Volkening JD, Mueni SN, Helmy MA, Afonso CL, Chaudhari PP, Decanini EL. Co-detection and genomic characterization of avian rotavirus A, avian orthoreovirus, and chicken megrivirus-C using nontargeted metagenomic surveillance in Indian broiler chickens. Front Cell Infect Microbiol. 2026 Feb 27;16:1690222. doi: 10.3389/fcimb.2026.1690222. PMID: 41835009; PMCID: PMC12982373.
19: Kim T, Volkening JD, Spatz SJ. Comparative Molecular Characterization of Three <i>Gallid alphaherpesvirus</i> Type 3 Strains 301B/1, HPRS24, and SB-1. Avian Dis. 2020 Jun;64(2):174-182. doi: 10.1637/0005-2086-64.2.174. PMID: 32550618.
20: Sharma P, Killmaster LF, Volkening JD, Cardenas-Garcia S, Shittu I, Meseko CA, Sulaiman LK, Joannis TM, Miller PJ, Afonso CL. Draft Genome Sequences of Five Novel Ochrobactrum spp. Isolated from Different Avian Hosts in Nigeria. Genome Announc. 2018 Mar 15;6(11):e00063-18. doi: 10.1128/genomeA.00063-18. PMID: 29545289; PMCID: PMC5854777.
21: Sharma P, Killmaster LF, Volkening JD, Cardenas-Garcia S, Wajid A, Rehmani SF, Basharat A, Miller PJ, Afonso CL. Draft Genome Sequences of Three Ochrobactrum spp. Isolated from Different Avian Hosts in Pakistan. Genome Announc. 2018 Apr 12;6(15):e00269-18. doi: 10.1128/genomeA.00269-18. PMID: 29650578; PMCID: PMC5897816.
22: Shittu I, Sharma P, Volkening JD, Solomon P, Sulaiman LK, Joannis TM, Williams-Coplin D, Miller PJ, Dimitrov KM, Afonso CL. Identification and Complete Genome Sequence Analysis of a Genotype XIV Newcastle Disease Virus from Nigeria. Genome Announc. 2016 Jan 28;4(1):e01581-15. doi: 10.1128/genomeA.01581-15. PMID: 26823576; PMCID: PMC4732329.
23: Shittu I, Sharma P, Joannis TM, Volkening JD, Odaibo GN, Olaleye DO, Williams-Coplin D, Solomon P, Abolnik C, Miller PJ, Dimitrov KM, Afonso CL. Complete Genome Sequence of a Genotype XVII Newcastle Disease Virus, Isolated from an Apparently Healthy Domestic Duck in Nigeria. Genome Announc. 2016 Feb 4;4(1):e01716-15. doi: 10.1128/genomeA.01716-15. PMID: 26847901; PMCID: PMC4742698.
24: Spatz SJ, Volkening JD, Ross TA. Molecular characterization of the complete genome of falconid herpesvirus strain S-18. Virus Res. 2014 Aug 8;188:109-21. doi: 10.1016/j.virusres.2014.03.005. Epub 2014 Mar 29. PMID: 24685675.
25: Spatz SJ, Garcia M, Riblet S, Ross TA, Volkening JD, Taylor TL, Kim T, Afonso CL. MinION sequencing to genotype US strains of infectious laryngotracheitis virus. Avian Pathol. 2019 Jun;48(3):255-269. doi: 10.1080/03079457.2019.1579298. Epub 2019 Mar 11. PMID: 30722676.
26: Spatz S, García M, Fuchs W, Loncoman C, Volkening J, Ross T, Riblet S, Kim T, Likens N, Mettenleiter T. Reconstitution and Mutagenesis of Avian Infectious Laryngotracheitis Virus from Cosmid and Yeast Centromeric Plasmid Clones. J Virol. 2023 Apr 27;97(4):e0140622. doi: 10.1128/jvi.01406-22. Epub 2023 Apr 6. PMID: 37022163; PMCID: PMC10134816.
27: Spatz S, Afonso CL. Non-Targeted RNA Sequencing: Towards the Development of Universal Clinical Diagnosis Methods for Human and Veterinary Infectious Diseases. Vet Sci. 2024 May 26;11(6):239. doi: 10.3390/vetsci11060239. PMID: 38921986; PMCID: PMC11209166.
28: Taylor TL, Volkening JD, DeJesus E, Simmons M, Dimitrov KM, Tillman GE, Suarez DL, Afonso CL. Rapid, multiplexed, whole genome and plasmid sequencing of foodborne pathogens using long-read nanopore technology. Sci Rep. 2019 Nov 8;9(1):16350. doi: 10.1038/s41598-019-52424-x. PMID: 31704961; PMCID: PMC6841976.
29: Volkening JD, Spatz SJ. Identification and characterization of the genomic termini and cleavage/packaging signals of gallid herpesvirus type 2. Avian Dis. 2013 Jun;57(2 Suppl):401-8. doi: 10.1637/10410-100312-Reg.1. PMID: 23901753. 30: Volkening JD, Spatz SJ, Ponnuraj N, Akbar H, Arrington JV, Vega-Rodriguez W, Jarosinski KW. Viral proteogenomic and expression profiling during productive replication of a skin-tropic herpesvirus in the natural host. PLoS Pathog. 2023 Jun 8;19(6):e1011204. doi: 10.1371/journal.ppat.1011204. PMID: 37289833; PMCID: PMC10284419.
31: Volkening JD, Spatz SJ, Garcia M, Ross TA, Maekawa DA, Rosenthal KS, Zamora AC, Skipper A, Blakey JR, Paudel R. A functional interleukin-4 homolog is encoded in the genome of infectious laryngotracheitis virus: Unveiling a novel virulence factor. PLoS Pathog. 2025 Jul 23;21(7):e1013219. doi: 10.1371/journal.ppat.1013219. Erratum in: PLoS Pathog. 2025 Dec 19;21(12):e1013791. doi: 10.1371/journal.ppat.1013791. PMID: 40700459; PMCID: PMC12327624.
